Anh Phan Nguyen

3 Questions 4 Answers 0 Followers

Questions related from Anh Phan Nguyen

QPCR for Knockout gene expression check?

Hi everyone, I am working on mice that having one of their genes knocked out I have to check and confirm the knock out by qPCR, so in qPCR, only the WT samples showed CT value while the KO samples...

19 August 2020 1,567 1 View

Media and coating condition for primary isolated mouse hepatocyte?

Hi everyone, I have two questions regarding mice hepatocyte, I would really appreciate it if anyone can help me. Currently, I am working on both immortalized and primary isolated mice...

21 November 2019 3,239 3 View

Stromal vascular cell adipogenic differentiation

Hi everyone, I have been struggling with the differentiation of mouse SVC cells into mature adipocytes lately. In the past, I have always isolated and cultured SVC in 15% FBS HG-DMEM and induced...

01 January 1970 7,410 2 View