Anh-Minh Tran

4 Questions 8 Answers 0 Followers

Questions related from Anh-Minh Tran

Is it okay to conduct a qPCR experiment with 10 µL of reaction when the Thermal cycler was calibrated for using 20 µL of reaction?

I tried to scale down the component in reaction (from 20 µL to 10 µL using Bio-Rad Universal SYBR Green) for saving material. I wonder if I can get precise results or not with a down-scaled...

13 December 2019 5,397 3 View

Is it acceptable to run a qPCR experiment with a minor gDNA contamination?

I have extracted total RNA from Lactobacilli and treated the samples with DNase I. When checking the -RT on qPCR, there was always gDNA contamination (appeared at cycle 33 or 35, automatic...

06 December 2019 9,995 3 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

23 August 2018 6,827 5 View

Question about qPCR: Can I apply the same E% value when using different concentration of primers?

I have performed the standard curve for my primer for qPCR in order for relative quantitative the expression level of the gene of interested. For standard curve, final concentration of each primer...

07 August 2018 4,770 9 View