Angita Shrestha

2 Questions 3 Answers 0 Followers

Questions related from Angita Shrestha

Avian influenza VLPs, do I harvest the cell lysate or supernatant for sucrose gradient VLP purification?

Hi, I am trying to make avian influenza virus VLP. I have a recombinant plasmid expressing HA,NA and M1 genes. I am using Drosophilla melanogaster S2 cells for these protein expression. When I do...

09 September 2018 6,392 2 View

How to detach chicken dendritic cells from the 6 well plate and label for flow cytometry?

Hi, I am trying to label chicken dendritic cells using Dec205 antibody for flow cytometry analysis. I make the DCs from bone marrow cells using IL4 and GMCSF. I also induce the cells with 200...

29 August 2018 7,559 5 View