Angita Shrestha

3 Questions 4 Answers 0 Followers

Questions related from Angita Shrestha

How do I reduce the non specific binding of hemagglutinin antigen to chicken bone marrrow derived DCs (BMDCs) for flow cytometry labelling?

I am trying to stain chicken bone marrow derived dendritic cells (BMDCs) with Hemagglutinin (HA) fused CD11c single chain fragment variable (scFv) antibody for flow cytometry. The staining with...

12 March 2019 702 4 View

Avian influenza VLPs, do I harvest the cell lysate or supernatant for sucrose gradient VLP purification?

Hi, I am trying to make avian influenza virus VLP. I have a recombinant plasmid expressing HA,NA and M1 genes. I am using Drosophilla melanogaster S2 cells for these protein expression. When I do...

09 September 2018 6,519 2 View

How to detach chicken dendritic cells from the 6 well plate and label for flow cytometry?

Hi, I am trying to label chicken dendritic cells using Dec205 antibody for flow cytometry analysis. I make the DCs from bone marrow cells using IL4 and GMCSF. I also induce the cells with 200...

29 August 2018 7,584 5 View