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I am working with J774A.1 cells purchased from ATCC on 1/16/18. I have worked with many different cell lines, but this one is turning out to be a little trickier for me. I'm looking for...
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I'm working on the cloning vector pET-15B, and I'm cleaving at the NdeI and XhoI restriction sites, and the only conceivable way I can think of is to run the product on a 5% agarose gel, which, if...
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