Angie Lessard

2 Questions 9 Answers 0 Followers

Questions related from Angie Lessard

Cell morphology of J774A.1 Cells and counting?

I am working with J774A.1 cells purchased from ATCC on 1/16/18. I have worked with many different cell lines, but this one is turning out to be a little trickier for me. I'm looking for...

22 January 2018 3,472 18 View

Is there a quick and easy way to find out if a double digest cut completely, when the portion I'm cutting out is only 7 bp?

I'm working on the cloning vector pET-15B, and I'm cleaving at the NdeI and XhoI restriction sites, and the only conceivable way I can think of is to run the product on a 5% agarose gel, which, if...

01 January 1970 3,141 3 View