Angelo Monteiro

2 Questions 2 Answers 0 Followers

Questions related from Angelo Monteiro

Will a PD-10 (Sephadex G-25) seperate a 10kDa molecule from a 2kDa?

Hello Everyone, I have got a technical question. I have ended doing a reaction in which both (2) starting materials have a 2kDa mass but I end up with a product of 10kDa mass. However, I use an...

15 July 2021 1,334 0 View

How to avoid oxidation of thiol, which has been previously reduced from disulfide, and consequently react it with a maleimide moiety?

I have this molecule which has thiols that are protected in a form of a disulfide with nitrobenzenethiol and I need to reduce them with DTT (cannot use PBu3 due to sensitivity of other groups) and...

09 May 2021 5,072 4 View