Angelo Guadagno

3 Questions 2 Answers 0 Followers

Questions related from Angelo Guadagno

What is the advantage to use both BSA and NGS for immunostaining?

I used Natural Goat Serum (NGS) for my immunostaining protocol. However I noticed that NGS is also combined with Bovine Serum Albumine (BSA). Is there any advantage to use both instead of NGS...

12 March 2024 4,693 2 View

How can I traslate the antibody incubation time and dilution from thinner to thicker brain sections?

I found the optimal antibody dilution and incubation time to stain cells of fish brain sections (50μm thick). However I need to stain those cells in thicker brain sections and I was wondering what...

12 March 2024 645 1 View

Does tissue clearing affect antibody labelling incubation time?

I am trying to establish a good protocol to stain neurons in whole fish brain. I know that tissue thickness affects the antibody incubation time, but I was wondering whether tissue clearing also...

06 December 2023 1,714 0 View