Can I use phosphate buffer saline instead of phosphate buffer in cryoprotectant with ethylene glycol and glycerol?

We store our brain tissue at -20°C (usually in 50% phosphate buffer, 30% ethylene glycol, and 20% glycerol). Would NaCl in the solution interfere with cryoprotection (i.e., would it allow freezing...

24 January 2017 8,376 1 View