Angel del Marco

3 Questions 5 Answers 0 Followers

Questions related from Angel del Marco

Which is the best method for neonatal mice tissue preparation for cryosectioning?

Hi everyone, We have difficulty sectioning neonatal brain tissue. We are following this protocol: We dissect the whole brain and use a 4% PFA fixation until several days. We do not perfuse the...

01 January 2020 6,750 4 View

Unusual annealing temperature of primers in rt-PCR??

Hi everyone, It may not be a problem really but...I was very surprised because although in manuals and from my experience, I know that the Ta can differ up to 5 degrees or more from the Tm of the...

11 November 2019 1,260 3 View

About sample processing for Amyloid-oligomer detection by ELISA?

Hi everyone, I am trying to detect Amyloid oligomers in brain tissue extract of APP mice by ELISA kit but the outcome is mostly undetectable. I am using IBL-Elisa kit: Amyloid Beta...

05 May 2019 5,606 2 View