Angel Martin Bastida

7 Questions 5 Answers 0 Followers

Questions related from Angel Martin Bastida

How to interpret PCA and cluster heatmap results?

Hi there. I have some data from RNA-seq experiments. In PCA, we observe that the dominating patterns in our data are explained by other variables, rather than by those we used to classify our...

12 July 2022 3,532 1 View

How to interpret samples grouping differently in PCA and heatmap?

Hi there, I have some data in which my samples don't cluster together in PCA according to the gruop of origin, but in a cluster heatmap they do. How should I interpret these findings? Thanks for...

11 July 2022 6,622 0 View

Sticky embryos during 0.1% PBST washings for IHC - should I supplement my washing media?

Hi there! I'm performing IHC experiments on PFA-fixed human day 5 embryos (blastocyst stage). After the staining steps, I perform washing in 0.1% Tween-20 in PBS. Although it is usually fine,...

08 April 2022 6,885 1 View

Convert Gene Ontology "name" to "ID"?

Does anyone know how to convert a list of GO-BP terms such as GO_RNA_CATABOLIC_PROCESS to GO-BP IDs such as GO:0006401? Thank you

25 March 2022 4,218 1 View

Active/cleaved caspase 3, expected nuclear or cytoplasmic signal in IF?

I am using active caspase 3 to detect apoptosis by immunofluorescence. In the last experiment, we got some positive signals in the cytoplasm. Can this be indicative of caspase activity? Thank you

14 March 2022 5,676 3 View

Contradictory Gene Ontology processes in RNA-seq?

Hi! I am conducting an RNA-seq experiment. After performing a functional enrichment analysis to identify significant biological processes, I have identified contradictory gene-ontology (GO) terms...

25 September 2021 3,526 5 View

Can someone help with TUNEL staining of human embryos?

Hi all, I am trying to stain the inner cell mass (ICM) of fixed human embryos with TUNEL to detect apoptosis (In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich) for confocal...

04 August 2021 3,653 2 View