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Questions related from Aneta Bragiel
I have been using ECL mix to detect the optimum primary antibody concentrations for a western blot and found out that even without the primary antibody I get bands though they are quiet light. The...
16 April 2014 2,228 3 View
I use PRB1-3 antibody from Santa Cruz and I am afraid it might not work well. The reason why I think this is that I get the same band pattern after the incubation with primary and secondary...
27 March 2014 10,049 2 View
I am doing CoIP to determine the interaction of my protein of interest with another protein. An antibody was used against 27kD protein to pulldown the protein complex and detection was done with...
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