Aner Ottolenghi

5 Questions 10 Answers 0 Followers

Questions related from Aner Ottolenghi

A good reliable cellular hypoxia assay?

I'm going to expose cells to hypoxic conditions, in order to establish the function of a synthetic promoter containing HRE's. For control sake, I want to see if cells actually developed hypoxic...

11 January 2017 6,588 3 View

Can I use a sandwich ELISA ab for WB? If so, which one? for the capture or the detection of biotin conjugated?

Read 3 answers by scientists to the question asked by Aner Ottolenghi on Oct 13, 2016

13 October 2016 8,688 4 View

Culturing CTLL cells with IL-2?

So my supervisor insists that you can culture CTLLs wih IL-2, instead of the ATCC recommended - Con A an T-STIM. It makes sense, after all propogation of these cells with IL-2 is the ED50 test for...

22 September 2016 1,691 3 View

MHC induction with Interferon gamma (IFNg)? What are the recommended amounts, and how long does it take?

Read 5 answers by scientists to the question asked by Aner Ottolenghi on Sep 20, 2016

20 September 2016 215 4 View

NFkB indution with TNFa in cell lines (Jurkat)?

What are the recommended quantities and duration until induction?

20 September 2016 7,514 4 View