I have thawed my cells (3T3 Fibroblasts) recently. Why am I seeing black pellet of cells after centrifuging during the subculturing?

I have thawed my cells 3T3 (fibroblasts) a couple days ago. After thawing I added 9ml of warm media into the cells and centrifuged them at 1000rpm for 5minutes. I sub-cultured my cells twice but...

17 January 2024 9,824 2 View