Andrzej Wierzbicki

2 Questions 6 Answers 0 Followers

Questions related from Andrzej Wierzbicki

Why are NIH 3T3 cells growing on the bottom in a soft agar transformation assay ?

I start soft agar transformation assay using NIH 3T3 cells. These cells shall not form any colonies in soft agar assay. In upper layer of agar these cells survived and start to proliferate (they...

06 June 2015 1,457 5 View

Why in soft agar transformation assay, are NIH 3T3 cells (which are not transfected) forming colonies?

I start to run soft agar colony formation assay. I used NIH 3T3 cells not transfected, transfected with few variants of LTK, empty vector as control and KRAS expression construct as positive...

04 April 2015 1,021 7 View