Andrew Michael Pickering

5 Questions 3 Answers 0 Followers

Questions related from Andrew Michael Pickering

Does anyone have experience of using an osmotic pump to deliver a plasmid into the mouse CNS?

I have seen a couple of papers on this but they tend to be rather old and not high impact. Does anyone have experience doing this? If so how effective is it? Do you get plasmid uptake by neurons?...

02 February 2018 1,212 0 View

Can you do Neomycin selection in a transfected cell line which already has a neomycin resistance construct inserted?

I want to transfect an already established transfected cell line with a construct of mine. The issue is that the cell line has been previously modified with a construct carrying a neomycin...

12 December 2017 5,820 5 View

Recommendations on a good stable transfected APP line for cytotoxcity assays?

We have a project using MC65 cells which are a Tet-OFF line for expression of the C99 fragment of APP. Tetracycline withdrawal produces cell death in this line within 2-4 days. We want to look...

12 December 2017 3,388 1 View

Can Streptomycin mimic Tetracycline?

I have been having trouble getting a published TET-OFF cell culture line to work. I have been finding that the treated and not-treated cells look pretty much the same. It occurred to me that...

11 November 2017 9,656 6 View

How much DNA can you load per well in a 2% acarbose gel without overloading?

I need to run 100 micrograms of DNA out on a gel to cut out and send off for purification as a prelude to microinjection. However I am concerned that adding this much will overload the gel and...

03 March 2017 8,386 9 View