Andrew Ronald Hagen

2 Questions 6 Answers 0 Followers

Questions related from Andrew Ronald Hagen

FlAsH-EDT2 non-specific labeling issue...does this reagent go bad?

I have been using FlAsH-EDT2 purchased from Carbosynth to develop a fluorescent probe that is basically a fluorescent protein (mCherry or mTurquoise2) with a tetracysteine motif at the C-terminus...

12 December 2016 8,463 0 View

Selective activation of a C-terminal lysine and not amino terminus?

I'm working with a water soluble 53 a.a. peptide that I would like to chemically conjugate to a carboxyl group using standard EDC/NHS coupling chemistry (I'm working in aqueous conditions)....

10 October 2016 8,763 6 View

Andrew Ronald Hagen

FlAsH-EDT2 non-specific labeling issue...does this reagent go bad?

Selective activation of a C-terminal lysine and *not* amino terminus?

Selective activation of a C-terminal lysine and not amino terminus?