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Questions related from Andrew D Beggs
Difficult question, but we're trying to carry out a PCR that produces a single stranded amplicon. We've tried using only the forward primer or the forward to reverse in a 500:1 ratio, without much...
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I am trying to design a set of primers to cover the coding region of a gene. I have looked at Exon Primer, but it doesn't really do what I want: 1) To design to strict size limits, i.e. the...
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Does anyone have a "simple" way of designing methylight RT-qPCR primers? I am looking to do this and don't have the money to fork out for Beacon designer. Ideally a method that could test the...
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I'm trying to amplify up a very GC rich (87%) region for pyrosequencing PCR. I'm getting lots of non-specific amplification - I've tried varying concentrations of DMSO (10%, 5%, 2.5%) , betaine...
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