Andrew Zikic

3 Questions 4 Answers 0 Followers

Questions related from Andrew Zikic

Frown/Unhappy face shaped bands in western blot?

Our lab recently switched from semi-dry to wet transfer system for western blotting. For some reason, our lower molecular weight bands always appear as frowns or unhappy face shapes (around the...

06 February 2014 406 12 View

Would an mRNA targeted by a morpholino stay intact? i.e. If the mRNA itself had a function, could this be conserved?

We are trying to figure out whether the mRNA of our gene of interest has a function. We want to inhibit protein translation but still allow for transcription of the RNA and stability of the RNA...

20 March 2013 631 2 View

Problem with MCF-10A cell appearance after a few days in culture

I recently switched from Human Recombinant Insulin to Insulin from bovine pancreas and my MCF-10A cells start to look weird after a few days in culture. They don't become 100% confluent, tend to...

25 February 2013 4,599 10 View