Andrew Whiteoak

4 Questions 7 Answers 0 Followers

Questions related from Andrew Whiteoak

How does one calculate the electrical resistance of the agarose gel?

I know that V=IR where V is voltage, R is resistance and I is the current in mA; so my problem is that the electrophoresis units available to me allow fellow researchers to change these parameters...

26 October 2013 5,222 5 View

Is there a specific melting temperature for wobbles?

To begin, I do know that Tm = 4(G+C)+2(A+T) So I don't need that to be reiterated. what I wonder is whether wobbles e.g. R = A/G Y = C/T K = G/T M = A/C W = A/T S = C/G B = C/G/T D = A/G/T H =...

14 October 2013 2,142 4 View

Is it possible to obtain 'wobbles' for DNA primers that simply cover either A/T only of G/C only rather than all four possibilities?

I am designing primers and would like to retain as much specificity as possible. However, due to variation in the gene I am coming across many issues where wobbles would need to intervene, and...

05 October 2013 9,502 15 View

How does one predict the melting temperature of DNA sequences?

A paper I've read says, "The melting temperature for primer regions should be 60-65c in the case of GC rich and 55-60c in the case of AT rich" (for LAMP sequencing.) I do not know how to predict...

02 September 2013 4,888 15 View