Andrew Wang

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Questions related from Andrew Wang

Can RNAlater help with freeze thawing tissue samples? Does RNAlater need to be reapplied to thawed tissue?

Hi there, I'm trying to perform RNA extraction with adipose tissues frozen in minus 80, which were treated with RNA later before freezing. Problem is there is a lot of tissue, much more than...

21 December 2023 7,150 0 View

How to present log2(fold change) from qPCR data on a graph and perform statistical analysis?

Hi there, I have qPCR results from 6 target genes and a housekeeping gene. Each different gene had its expression measured after 3 different treatment conditions and a non treatment control. I...

26 September 2023 2,573 2 View

Can we use RT-qPCR results from different 96-well plates if the samples are the same?

So I was running an RT-qPCR, but as you can see from the attachment some of my technical replicate triplets have CT values that vary by 1 (e.g. 26.16, 25.88, 25.14), leading to standard deviation...

07 September 2023 6,815 7 View

Is there a difference between the standard curve dilution concentrations in a sandwich ELISA vs indirect ELISA?

We have bought a detection antibody and used it to run an indirect ELISA. Initially, we were not getting meaningful readings when diluting the recombinant standard sample to the picogram level, so...

04 September 2023 8,012 2 View

How do I calculate confidence intervals (error bars) for fold change and log 2 fold change for qPCR?

I have heard that it is better to show PCR gene expression results via log 2 fold change rather than just fold change, but how does one calculate the confidence intervals (error bars) for...

06 July 2023 755 3 View