Andrew Varley

4 Questions 9 Answers 0 Followers

Questions related from Andrew Varley

What is the impact of pH on LiCl precipitation of RNA?

I need to precipitate lots of RNA from a 10 mM MES pH 6 solution. I'm wondering if its essential to add Tris to adjust the pH to 8 for successful LiCl precipitation? Thanks!

10 January 2023 8,517 2 View

What is the best stain for short RNA oligos (12-mer)?

Hi, I'm trying to visualize ssRNA 12-mers on UREA-PAGE with EtBr or GelRed stain; however, I cannot see any bands. In contrast, both EtBr and GelRed seem to detect ssRNA 21-mers without any issue....

17 May 2021 5,572 4 View

I'm having difficulty adding a ssDNA poly-A tail to my dsDNA using terminal transferase and ATP. Has anyone ever had success doing this?

I'm using NEB's Terminal Transferase (https://www.neb.com/products/m0315-terminal-transferase). I have tried using more enzyme, and tried using more ATP in reactions with no noticeable success. My...

11 August 2016 7,699 4 View

How can I cast a vertical agarose gel for protein electrophoresis?

I'm trying to identify a novel enzyme using a zymograph (SDS-PAGE with the enzyme's substrate in gel. The proteins are renatured then incubated to degrade the substrate. Staining identifies its...

16 December 2015 4,337 3 View