Andrew Silvanus

2 Questions 4 Answers 0 Followers

Questions related from Andrew Silvanus

How to design allele specific primers?

Hi, I am trying to design allele specific primers for a mouse locus with SNP's from B6.Cast. Does anybody have an expertise in designing such primers?.

01 February 2015 7,335 7 View

Can anyone suggest me solutions for a problem with lentivirus production and infection?

I am trying to infect mouse embryonic stem cell(Ezh2-/-) with pLVX-ef1 alpha-ires-puro vector from clontech. I am using second generation packaging virus for lentivirus production. I transfected...

09 January 2014 4,157 5 View