Andrew Herppich

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Questions related from Andrew Herppich

Copper causing absense of signal in CuAAC?

I have been struggling with CuACC in HeLa lysates for months. I am using an activity-based alkyne probe which becomes attached to proteins. I click the tag to biotin-azide for visualization on a...

07 February 2025 7,904 0 View

I have an SDS-PAGE and western blot issue that has me, my colleagues, and my PI stumped?

I have been having issues with my Streptavidin-Cy5 blots. When I run the SDS-PAGE, electrotransfer, and blot with SA-Cy5, random bands go across the entire gel, even across empty lanes. I have...

30 December 2023 4,385 0 View