Andrew Ronald Hagen

2 Questions 5 Answers 0 Followers

Questions related from Andrew Ronald Hagen

Expressing (but not purifying) oligopeptides in E. coli: Solubility/Expression questions?

I would like to express some short, ~20 residue, ~1.2 kDa oligopeptides in E. coli where they will have an in vivo function. I do *not* need to purify them (which seems to be the concern of most...

09 February 2018 5,670 0 View

Selective activation of a C-terminal lysine and not amino terminus?

I'm working with a water soluble 53 a.a. peptide that I would like to chemically conjugate to a carboxyl group using standard EDC/NHS coupling chemistry (I'm working in aqueous conditions)....

04 October 2016 2,556 6 View

Andrew Ronald Hagen

Expressing (but not purifying) oligopeptides in E. coli: Solubility/Expression questions?

Selective activation of a C-terminal lysine and *not* amino terminus?

Selective activation of a C-terminal lysine and not amino terminus?