Andres Leal

4 Questions 9 Answers 0 Followers

Questions related from Andres Leal

Sample preparation of OMVs for SDS-PAGE?

Hi everybody! I'm workin with outer membrane vesicles from E. coli (OMVs) and I want make SDS-PAGE, however I can´t see nothing in Coomassie staining. I use this protocol: 1. OMVs are isolated...

07 July 2017 9,141 5 View

OMVs conjugations with FITC?

Hi everyone!.. I'm trying of conjugate OMVs (Outer Membrane Vesicles) with FTIC (1mg/mL). I incubated 1h (RT) ratios 1:1 of OMVs (Resuspended in PBS) with FITC. Then, I pelleted to 17000rpm/4°C...

07 July 2016 5,310 3 View

Can anyone advise me on seeding cells in microplate P96?

Hello... I have worked many years with cells but recently, I have had a doubt!!!... If I have a cellular suspension of 1x106cells/mL and I take 100 uL, I have 1x105 cells in this volume?......

01 January 2016 682 5 View

When working with OVMs, do I heat the cells?

Hi everyone!!... I work with outer membrane vesicles (OMVs) for to evaluate the immune response in J774A.1 macrophages-like. The OMVs are suspended in PBS and I diluted in RMPI for test in the...

01 January 2016 964 1 View