Andres Leal

3 Questions 5 Answers 0 Followers

Questions related from Andres Leal

Sample preparation of OMVs for SDS-PAGE?

Hi everybody! I'm workin with outer membrane vesicles from E. coli (OMVs) and I want make SDS-PAGE, however I can´t see nothing in Coomassie staining. I use this protocol: 1. OMVs are isolated...

21 July 2017 3,027 5 View

OMVs conjugations with FITC?

Hi everyone!.. I'm trying of conjugate OMVs (Outer Membrane Vesicles) with FTIC (1mg/mL). I incubated 1h (RT) ratios 1:1 of OMVs (Resuspended in PBS) with FITC. Then, I pelleted to 17000rpm/4°C...

27 July 2016 5,239 2 View

How can I permeabilise S. cerevisiae for IFI?

Hi everyone!! I never have worked with S. cervisiae and need to permeabilise for IFI... Is it possible or I need to induce spheroplast? Thanks...

18 December 2015 4,256 1 View