Andreia Vargas-Seymour

2 Questions 6 Answers 0 Followers

Questions related from Andreia Vargas-Seymour

Can't clone cDNA insert (797bp) into pET22b with T4 DNA ligase - what am I doing wrong?

I've been trying to ligate this insert into pET22b with no success. I have tried molar ratios of 3:1 and 1:1 (insert:vector). Both the vector and the insert (within pUC57) are double-digested with...

01 January 2014 561 17 View

Osmotic shock - any suggestions?

I've been looking for a protocol to purify overexpressed proteins in the periplasm of E. coli BL21(DE) Star. Have you used any osmotic shock protocols you would recommend? I've found this one from...

01 January 2014 6,922 2 View