Andreas M Kist

3 Questions 8 Answers 0 Followers

Questions related from Andreas M Kist

Are there any papers or websites guiding someone through the proper design of floxing genes?

Hi all, I am wondering if there's any paper or website with detailed information how to design a floxed gene, i.e. how to position your loxp sites, such as in the introns, avoid this and this...

05 May 2015 4,379 3 View

When eluting from Magnetic Protein A/G beads with SDS sample buffer, do I have to use DTT?

I'd like to investigate if my protein of interest is covalently bound to the protein which was caught by the antibody. Can I leave the elution buffer (which is my SDS sample buffer) without DTT?...

10 October 2013 7,265 4 View

Can I use HisProbe-HRP with milk instead of BSA?

I bought the HisProbe-HRP system from Thermo. First experiments showed very very nice bands and it is much more sensitive than anti-6xHis-Antibodys. But it is recommended using BSA as a blocking...

09 September 2013 4,402 2 View