Andreas Blees

2 Questions 3 Answers 0 Followers

Questions related from Andreas Blees

How do I lyse HEK293 freestyle cells?

I want to prepare crude membranes from HEK293freestyle cells. Since I just recently started to work with this cells, I'm not sure what is the best way to crack the cells. I would either use a...

02 February 2015 4,394 4 View

Can someone suggest which vector to insert into a long PCR product?

I want to insert a long PCR product (about 5000bp) into a vector. I do have the right PCR product, but every attempt to insert the product into pcDNA3.1 or pIRES2-eGFP failed so far. I am not sure...

07 July 2013 4,890 14 View