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Hi. When I purify my His-tagged membrane protein with IMAC, I label the protein (containing a single cysteine) with fluorescein-5-maleimide while the protein is immobilized to the Ni2+ NTA. I...
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Hi. I work with a bacterial amino acid transporter into which I have introduced a cysteine. Hence, I would like to conjugate fluorescein-5-maleimide to this cysteine. Unfortunately, the degree of...
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