Andrea Pirosu

3 Questions 3 Answers 0 Followers

Questions related from Andrea Pirosu

How can I purify nuclei from liver for atac-seq?

Dear All, I have recently started to work on ATAC-seq and for this purpose I need nuclei as pure as can be prior to transposition and so on. After homogenization by douncing. I am actually using...

01 January 2018 1,836 0 View

Why am I not able to resolve the smear on agarose gel after chromatic shearing?

Hey people, I am struggling since some months with a problem: I work with ChIP from liver tissue, and every time I have to check size on the agarose gel I always see a sharp band but not a...

12 December 2017 7,531 0 View

Staining not understandable background: where was the mistake?

Hi to everybody! I am struggling with Silver Staining, I am preparing a BSA curve but I see often bands around 20 kDa that are not expected and I have a strange gel background as you can see from...

01 January 2017 2,905 1 View