Andrea Frapporti

2 Questions 6 Answers 0 Followers

Questions related from Andrea Frapporti

DNA FISH: nick translation on purified PCR product (linear)?

Hi everyone, I want to carry out some DNA FISH experiments on different genomic regions ranging from 12 to 19 Kb in length. My strategy would be to first amplify them by PCR and use the purified...

12 December 2018 4,351 4 View

8% PFA stock solution: which protocol?

Hello everyone, I currently use 8% PFA solution for my immunofluorescence experiments. Before, I simply used to heat the powder in the corresponding volume of water, then filtered it and stored...

01 January 2015 5,920 17 View