Andrea Carobbi

5 Questions 9 Answers 0 Followers

Questions related from Andrea Carobbi

Is there a limit of the plasmid size that can be transferred by triparental mating?

Hello colleagues, I'm trying to transfer the plasmid pKEB31:GOI carried by E. coli DH5alfa on Pantoea agglomerans using E. coli pRK2073 as helper. I did this triparental mating lots of time with...

10 October 2019 302 4 View

Why my mutant lose the vector so fast?

I create a mutant deleting a GOI and I complemented it using a vector carrying the removed GOI. I checked my strains by PCR using two pairs of primer, a pair for the removed gene and a second pair...

09 September 2019 294 5 View

Why I can't complement my knockout mutant?

I have knocked out a gene in my bacteria (deletion by suicide vector) and I can observe a phenotype for that mutation. Then I inserted the same gene in a vector and transformed in E.coli which I...

09 September 2019 5,884 3 View

Why I got DNA amplification in NTC and -RT samples (qPCR)?

Hi everybody, I carried out a qPCR in order to quantify expression level of two genes under different conditions. I used three pairs of primers, one for the endogenous control and two for the...

08 August 2019 7,633 6 View

How to determine the concentration of a root extract?

Hello everyone, I made a beetroot extract I need to use in my experiment but to be sure to use the same concentration each time I need to have a standard to compare it. I thought to determine...

12 December 2018 7,379 4 View