Culturing THP-1's with pyruvate and essential amino acids or without, that is the question?

I culture THP-1 cells in RPMI (10% FBS, 1% Pyruvate, 1% non essential amino acids and 1% Pen/Strep). I have been differentiating the THP's into neural macrophages, however I have had 2 batches of...

05 June 2023 7,753 1 View