Andras Donaszi-Ivanov

3 Questions 6 Answers 0 Followers

Questions related from Andras Donaszi-Ivanov

Is anyone familiar with normalization of qRT-PCR data of miRNA levels isolated from exosomes?

Dear All, I'm having trouble figuring out how best to normalize this sort of data. The exosomes were isolated from tissue culture medium, and reading the literature I can find spiked in external...

23 September 2014 8,085 8 View

What are the best in vivo cell viability assays for virus work?

I would like to monitor cell viability during an experiment (in a quantitative way if possible) using the very same cells I'm using for the experiment. I'd be using poly IC and viruses, and would...

28 March 2014 4,446 6 View

How does siRNA work in a Dicer-knockout cell line? Also: how does suppression/cleavage decision work in practice?

1. More specifically: what loads the siRNA onto RISC when Dicer is not present? I do know siRNA works quite well without Dicer; I just can't find any information on RISC loading in systems where...

06 November 2012 2,735 3 View