Ancy Thomas

3 Questions 37 Answers 0 Followers

Questions related from Ancy Thomas

Any reference for analysis of total free amino acids in a cell extract using HPLC with C18 column and UV detector in biphasic system?

I am interested in the profiling of total and free amino acids from unicellular algae

11 November 2015 2,284 4 View

Can someone suggest how to improve efficiency of protein binding during purification?

Dear all, I am trying to purify one of the recombinant protein expressed in E.coli with an N-terminal 6X HIS tag. As it accumulates in inclusion body, I am compelled to solubilize it with urea or...

10 October 2012 4,089 17 View

Protein expression in yeast Hi friends, we are facing problem in expressing a viral envelope protein in yeast P.pastoris.

(This viral gene has an N terminal hydrophobic transmembrane domain). We could successfully clone and generate positive yeast transformants using pPICZ alpha secretory vector. It is made sure that...

10 October 2011 9,630 3 View