Anchang Charles Gwellem

10 Questions 8 Answers 0 Followers

Questions related from Anchang Charles Gwellem

Can HOMER be used to predict the binding site of a transcription factor on a DNA sequence?

I wish to find out if HOMER tool can be used to predict the binding site of a transcription factor on a list of DNA sequences. Thank you in advance for your kind and helpful responses.

28 May 2019 8,797 3 View

How do I computationally predict the binding position of a DNA binding protein on the promoters of differentially expressed genes?

I have a list of genes that have been proven to be up-regulated in a human disease condition of interest. I also have a protein which according to literature is said to be a DNA binding protein. I...

26 May 2019 1,914 2 View

Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?

I have a list of differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a...

21 February 2019 4,862 1 View

Data base or computational tool to help predict if two predicted interacting surface proteins are located on separate cells or on the same cell?

Is there any computational method or data base with the help of which one could predict if two interacting cell surface proteins are located on separate cells or are located on the same cell surface?

29 January 2019 9,519 1 View

Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?

Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially...

24 January 2019 7,852 3 View

How do I identify which differentially expressed genes are cell surface genes in mice?

Hi colleagues. I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. In a previous study from my research group, it was identified that two...

23 January 2019 6,587 1 View

Why this change in morphology in endothelial colony forming cells (ECFC)?

Dear colleagues, I culture on 2% PAN-biotech gelatine, cord blood MNCs into endothelial colony forming cells (ECFCs), using Endopan 3 kit medium. For many of my samples, I obtain a change in...

18 April 2017 721 2 View

Time interval to split endothelial cells?

Dear colleagues, Does anyone has an idea on the optimal time interval between splitting one endothelial cell culture passage and the next? Thank you in advance.

22 August 2016 6,634 4 View

DMEM or EGM-2 for culture of HUVECS?

Hello ladies and gentlemen...Can HUVECS also be optimally cultured in DMEM or EGM-2 media? Which of these two is better?

20 July 2016 1,847 2 View

What is the maximum number of ECFCs (endothelial colony forming cells) that can be frozen in one vial?

Assuming the use of 90%FCS+10% DMSO as cryoprotectant and 1ml of final volume. Thank you in advance

16 May 2016 5,477 3 View