Anchal Nigam

2 Questions 9 Answers 0 Followers

Questions related from Anchal Nigam

A working protocol for smRNA FISH in breast tissue (fresh frozen/FFPE))?

I have been trying to work on smRNA FISH in breast tissue samples with probes and buffers from Stellaris (Biosearch technology). Unfortunately, I am not getting any signal in either of the...

15 September 2019 8,122 2 View

How can one ensure ligation of a 300bp fragment into 5369bp host?

My plasmid carrying the insert is 5698bp and my host plasmid is 5737bp. I had done Xba1 and Sac1 digestion for both and had dephosphorylated my host. I had done the ligation in the molar ratio...

19 October 2014 5,183 15 View