Why am I getting low concentration of DNA plasmid after DNA extraction from clones?

DNA is about 8kb, cells: XL1-blue, selection: ampicillin, PCR screening of clones: Encyclo Plus PCR Kit (Evrogen), DNA exstraction: Plasmid Miniprep (Evrogen). Site-directed mutagenesis per...

27 May 2021 7,072 8 View