Anass Fendrhoussi

2 Questions 5 Answers 0 Followers

Questions related from Anass Fendrhoussi

Using the CRISPR/Cas9 system to knock out a gene, but I am stuck on how and where to insert my sgRNA/gRNA?

We are working with E.coli K12 strain and want to knock out a certain gene in its genome. We already have some concepts of sgRNA, but we do not know in which plasmid (either pCas9 or pGRB or...

10 September 2018 7,774 0 View

Is there an alternative for EDTA when using it in purification of my his-tagged protein in either Ni-NTA or Cobalt column?

I am trying to search for a better way to purify my histidine tagged protein by looking for a replacement for EDTA, since it trips the metal ions in de IMAC columns. I first used the Nikkel column...

27 January 2018 1,968 10 View