Anas Fadli

3 Questions 1 Answers 0 Followers

Questions related from Anas Fadli

Which gene sequences to use for primer design in order to validate RNAseq Differential Gene Expression by qPCR?

I would like to validate the results of RNAseq but I am not familiar with this. I currently have a table with Gene IDs (Ciclev10004574m.g for example) and their Arabidopsis homologs (AT5G52300.1...

30 November 2021 8,507 2 View

Can RNA to cDNA reverse transcription amplify the amount of nucleic acids in the sample?

I am trying to convert RNA to cDNA in order to quantify gene expression. I started with 50 ng/uL RNA and I obtained 1500 ng/uL nucleic acid at the end (I am not if it's only cDNA or cDNA+remaining...

05 November 2021 1,951 3 View

I have a dataset that shows the segregation of a SSR marker (only 1) in 36 hybrid citrus rootstocks. Can I use AMOVA to analyse molecular variance?

The objective of the analysis is to compare between offsprings and parents and to identify hybrids (test of hybrid purity).

10 December 2018 1,161 4 View