I am doing a fluorescence-based membrane potential on CFTR using forskolin, but the fluorescence values are nearly identical to DMSO, any insights?

I am running the assay on Caco-2 cells using a micro-plate reader. I used the DiSBAC2(3) dye, with excitation and emission wavelengths of 530 nm and 560 nm. I am seeding the cells into 6-well...

26 September 2024 7,255 0 View