Ananda Baral

3 Questions 10 Answers 0 Followers

Questions related from Ananda Baral

Coagulation Problems in Rat Primary Hepatocyte Isolation?

Hi all, I am currently working on rat hepatocyte isolation.I use HBSS(Ca2+ free) from sigma(9.5g/L) as perfusion medium via cannulation of portal vein.Then in about 2 min once the liver gets...

03 July 2018 4,097 0 View

Why do Cell Viablity have to be over 80 % for experimentation after isolation of primary cells?

I am working with rat hepatocytes isolation. I use a 2 step collagenase perfusion for it. However,often the viability is not good-only around 70 %. So, I am wondering is there any significant...

01 January 1970 1,363 8 View

What is the difference between Regular Autophagy and Defective/Dysfunctional Autophagy in Molecular Level?

What is the difference between Regular Autophagy and Defective/Dysfunctional Autophagy in Molecular Level? Most importantly what does it even mean at molecular level other than the meaning we can...

01 January 1970 224 5 View