Anamika Singh

3 Questions 3 Answers 0 Followers

Questions related from Anamika Singh

Vectors with the Chitin Binding Domain (CBD)?

I would like to introduce the CBD as an affinity tag at the C-terminus of my construct. For that which CBD gene do I need to look at. So that I can purify my construct later on with Chitin beads....

13 June 2021 355 3 View

What is the method getting an "apparent Kd" for protein-protein interaction from pull down assay or titration assays?

I am planning to do titration assay with purified His-tagged protein with biotinylated protein (purified). I need more details about getting an "apparent Kd" for interacting proteins.

13 June 2017 1,323 3 View

Recombinant Protien purification

hi all actually i am facing problem while purifying rec. protein of plant origin.The protein is coming in pellet. i tried to solubilise it, it works but problem of refolding still persist i.e....

01 December 2010 9,489 1 View