Anamaria C. Daza-Sanchez

3 Questions 3 Answers 0 Followers

Questions related from Anamaria C. Daza-Sanchez

Counting cells attached to Cytodex 3 Microcarriers with Presto Blue ?

Hi! Has anyone followed the growth of cells attached to Cytodex 3 microcarriers using Presto Blue assay? Could you please provide me a detailed protocol? I've had some difficulties with...

07 October 2020 1,188 4 View

How can I follow the growth of MSC in microcarrier suspension-culture?

I have 50 mL of volume at the beginning of the culture and I just can take samples of 1 mL per day, so there are very few cells to count them using trypan blue (after washing with PBS and...

05 July 2019 721 4 View

Have you had problems amplifying by pcr fragments containing AAV-ITR? How can I improve my pcr reaction?

Im trying to amplify a fragment that include ITRs of AAV, from plasmid. But i have a lot of problems.... And i have problems trying to sequence ITR, we use Macreogen service, any suggestion? Regards

30 July 2015 335 3 View