Anam Nayab

2 Questions 12 Answers 0 Followers

Questions related from Anam Nayab

Does addition of DTT to the protein sample favours crystallization inhibition?

I am purifying a protein having zinc fingers using pet28a his tag.During induction I add Znso4 to the LB media.The protein has 8 cysteine residues and pI is 6. I have tried purifying my protein...

11 November 2018 8,125 0 View

Problem purifying my protein after cutting its sumo tag

hi, i am trying to purify an 8kda protein.PI 6.95, HIS sumo tagged. after adding sumo protease to my elution sample plus 2mM DTT .DAILYSIS BUFFER being 20mM NaH2PO4 500mM NaCL..when i again bind...

05 May 2017 529 10 View