Anam Farooqui

3 Questions 3 Answers 0 Followers

Questions related from Anam Farooqui

Why uninduced sample is used as a negative control in protein expression results? why not neat vector i.e. without any cloned gene in a vector ?

what is the logic behind using an uninduced sample as a negative control in protein expression experiments? why cant we use neat vector???? I'm getting bands at the expected size on SDS PAGE of my...

05 May 2018 7,642 2 View

At what stage total RNA should be isolated from bacterial cell??from overnight grown primary culture or secondary culture??

I want to study the quantitative gene expression by real time PCR. For this i need to isolate total RNA and then do cDNA synthesis. I'm little confused that we should harvest the bacterial cells...

03 March 2018 1,424 6 View

Why anti-His is binding at non specific site in my protein?

I have cloned my gene of interest HBV surface gene in pRset B expression vector(prokaryotic). I have cloned 1273 bp in pRset B vector but the stop codon is present 681st nucleotide. So ideally the...

03 March 2018 1,900 4 View