Problem purifying my protein after cutting its sumo tag

hi, i am trying to purify an 8kda protein.PI 6.95, HIS sumo tagged. after adding sumo protease to my elution sample plus 2mM DTT .DAILYSIS BUFFER being 20mM NaH2PO4 500mM NaCL..when i again bind...

19 May 2017 9,711 9 View