Anakha Satish

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Questions related from Anakha Satish

Is it possible to cut ligase mix from agarose gel for PCR afterwards?

My experiment involved ligation of DNA size 800 bp (which was amplified from ligated DNA sized 400 bp each) with DNA size 400bp to create fragment of size 1200bp. I did electrophoresis of the...

06 April 2023 7,581 3 View

Is it normal for plaque size to differ on one agar plate after infection with helper phage M13K07? If not, how can i solve this issue?

The plate is made with 2TY with Ampicillin, Kanamycin and Glucose. The plaques that form are small and big on the same petri dish, its got a pretty disproportionate look to it.

01 September 2021 5,076 3 View