Anabel-Lise Le Roux

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Questions related from Anabel-Lise Le Roux

Can I perform a double transfection of siRNA and plasmid for fluorescent protein expression?

I would like to transfect my cells with a plasmid for a fluorescent protein (classical membrane marker I normally transfect via electroporation) AND transfect a siRNA to silence another protein....

13 January 2022 3,483 8 View

Does anyone have information on lateral pressure of a supported lipid bilayer obtained by liposome deposition?

I am currently working with liposome deposition of DMPC-DMPG 2:1 on different substrates (glass and equivalent L1 chip of biacore). I am not sure whether I am obtaining fully fused bilayers, I...

22 March 2015 9,912 6 View

Can anyone give me tips for obtaining a supported lipid bilayers on glass surfaces?

I am using the liposome deposition technique of DMPC/DMPG liposomes on glass microscope slides or cover slits. I am working in a phosphate buffer at ionic strength 50mM and pH7. I would like to...

25 March 2014 9,858 14 View

Anyone familiar with EGFP concentration measurements using absorption at 488nm?

I would like to measure the concentration of a mixture of two proteins. I cannot use the absorption at 280nm because I do not know the ratio between both proteins. But one of them has a eGFP...

16 February 2014 3,052 5 View