Anabel-Lise Le Roux

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Questions related from Anabel-Lise Le Roux

Can I perform a double transfection of siRNA and plasmid for fluorescent protein expression?

I would like to transfect my cells with a plasmid for a fluorescent protein (classical membrane marker I normally transfect via electroporation) AND transfect a siRNA to silence another protein....

13 January 2022 3,473 8 View

Can anyone give me tips for obtaining a supported lipid bilayers on glass surfaces?

I am using the liposome deposition technique of DMPC/DMPG liposomes on glass microscope slides or cover slits. I am working in a phosphate buffer at ionic strength 50mM and pH7. I would like to...

25 March 2014 9,849 14 View

Anyone familiar with EGFP concentration measurements using absorption at 488nm?

I would like to measure the concentration of a mixture of two proteins. I cannot use the absorption at 280nm because I do not know the ratio between both proteins. But one of them has a eGFP...

16 February 2014 3,040 5 View