Anabel-Lise Le Roux

3 Questions 18 Answers 0 Followers

Questions related from Anabel-Lise Le Roux

Can I perform a double transfection of siRNA and plasmid for fluorescent protein expression?

I would like to transfect my cells with a plasmid for a fluorescent protein (classical membrane marker I normally transfect via electroporation) AND transfect a siRNA to silence another protein....

13 January 2022 3,478 8 View

Can anyone give me tips for obtaining a supported lipid bilayers on glass surfaces?

I am using the liposome deposition technique of DMPC/DMPG liposomes on glass microscope slides or cover slits. I am working in a phosphate buffer at ionic strength 50mM and pH7. I would like to...

25 March 2014 9,853 14 View

Anyone familiar with EGFP concentration measurements using absorption at 488nm?

I would like to measure the concentration of a mixture of two proteins. I cannot use the absorption at 280nm because I do not know the ratio between both proteins. But one of them has a eGFP...

16 February 2014 3,044 5 View