Ana Paula Sato

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Questions related from Ana Paula Sato

Does anyone have a protocol to share on how to use the Chelex 100 resin (Bio-rad) to chelate calcium from a cell culture medium?

I work with cell culture of keratinocytes and from the second passage onwards I have to reduce the amount of calcium in the culture medium. Does anyone have a protocol on how to use the Chelex 100...

12 February 2024 9,389 1 View

What is the best concentration of epidermal growth factor that I should supplement in the keratinocyte cultivation medium?

I will work with bovine keratinocytes and I will supplement the culture medium with Epidermal Growth factor (EGF) and Bovine Pituitary Extract (BPE). I read some papers that this amount can vary...

18 September 2023 6,134 0 View