Ana Paula Sato

4 Questions 4 Answers 0 Followers

Questions related from Ana Paula Sato

Has anyone worked with double immunofluorescence staining of primary fibroblast cells?

Hello everyone, I am trying to perform double immunofluorescence (IF) staining in bovine primary fibroblasts using anti-vimentin and anti-cytokeratin antibodies to characterize the cells. I...

4 weeks ago 1,202 3 View

How can I increase my RNA yield and 260/230 ratio- Qiagen RNeasy Mini Kit?

Hello everyone! I'm looking to extract high quality RNA from frozen primary Fibroblasts (~1x10^5 cells) in order to do transcriptomics analysis. My RNA yield is low, variable from 11 to 40ng/ul...

03 September 2024 1,217 8 View

Does anyone have a protocol to share on how to use the Chelex 100 resin (Bio-rad) to chelate calcium from a cell culture medium?

I work with cell culture of keratinocytes and from the second passage onwards I have to reduce the amount of calcium in the culture medium. Does anyone have a protocol on how to use the Chelex 100...

12 February 2024 9,457 1 View

What is the best concentration of epidermal growth factor that I should supplement in the keratinocyte cultivation medium?

I will work with bovine keratinocytes and I will supplement the culture medium with Epidermal Growth factor (EGF) and Bovine Pituitary Extract (BPE). I read some papers that this amount can vary...

18 September 2023 6,182 0 View